Population study of the  Danube sterlet

(Acipenser ruthenus L.)

 

 * Kolarevic, J., **Lenhardt, M., **Cakic, P. and  *Jaric,I.

 

* Centre for multidisciplinary studies, Kneza Viseslava 1, 11000 Belgrade

** Institute for Biological Research, 29 Novembra 142, 11000 Belgrade

 

 

INTRODUCTION

 

Sterlet (Acipenser ruthenus L.) is the smallest species among the Danube sturgeons. It is a potamodromous resident species. However, in spring it leaves for flooded areas in quest for food and place where they can reproduce (Billard and Lecointre, 2001).

Sterlet is particularly intriguing due to its wide dispersal range and high variability. In contrast to other acipenseriformes, which belong mainly to one out of nine biogeographic provinces in which sturgeons occur, sterlet spawns in three provinces, on two continents (Bemis and Kynard, 1997) and has different history patterns in different river systems.

In first half of XX century, Russian ichthyologist and biogeographer, Leo Semenovich Berg, recognized two morphs within European sterlet. After that, many contributions were published for and against existence of two sterlet morphs and today these questions are still open.

Together with all other sturgeon species, sterlet is listed in Nature 2000 list, IUCN Red List of Threatened species, Convention on the Conservation of Migratory species of wild animals, Convention on the conservation of European wildlife and natural habitats and CITES convention. Unfortunately this preservation effort was not very fruitful considering that most of the sturgeon populations are still declining.

Bearing in mind, that intensive catch, especially of younger juvenile sterlet specimens, exists from the middle of XX century and that it was followed by habitat destruction and water pollution in Serbian part of the Danube River, at the beginning of 2002, we started a complex investigation on natural sterlet population in the Danube near Belgrade.

This research is done as a part of a project sponsored by Ministry of Science and Technology of Republic of Serbia and it is carried out by Centre for Multidisciplinary Studies in Belgrade.

Our main goal is to learn as much as we can about this endangered species and its habits.

 

MATERIAL AND LOCATION OF RESEARCH

 

Material, which consisted of 500 sterlet specimens was gathered monthly in the Danube River during years 20002 and 2003. Fish was caught using modified drag nets on three localities: Zemun (1173km), Visnjica (1162km) and Oresac (1123km). Specimens were than submitted to the series of different analysis, which included morphological analysis, age and sex determination, growth rate analysis, histological analysis, biochemical analysis, blood film analysis and parasitological analysis.

 

 

METHODS AND RESAULTS

 

Morphometrical analysis

We have conducted broad morphometrical analysis, in attempt to resolve question about existence of two sterlet morphs. Beside weight and total length, 24 different morphometric and 3 meristic variables were used for fish comparison, 15 of them in head region. Acquired data were tested by detailed statistical analysis. As a result, some indications in favor of existence of two sterlet forms have already appeared, but further data analysis are expected to give more precise answers. Genetic analysis of sterlet fin samples will be done at the University of Ferrara, Italy.

We believe that the use of more sophisticated statistical methods for analysis of gathered data, together with genetic analysis of sterlet populations, will offer better understanding of this problem.

 

Age structure

Altered Stevenson and Secor method (1999) was used In order to determine age structure of sterlet populations. Pectoral-fin spine sections approximately 2 mm width were removed from gathered specimens, mounted on glass slides, polished and watched under microscope. According to all 500 analyzed sterlet specimens, almost all were in their first or second year of life cycle.

 

Growth rate

Length and weight growth was monitored on monthly basis, in order to determine variations in growth rates during different seasons. Special attention was given to the comparation of results from different localities and to the differences between existing age classes. Length – weight relationship and condition factor were calculated and used as good indicator of water pollution level and food availability.

 

Sex determination

Sex was determined using histological analysis of gonad segments. Gonads were fixed in Bouin’s fixative, dehydrated, lightened in xylol and molded in paraffin. Tissue sections (5 mm) were cut and stained with haematoxylin and eosin (Lenhardt and Cakic, 2002). Results of examination showed that nearly all gathered specimens were reproductively immature.

 

Histological analysis and biochemistry

Histological analysis of gills, liver, kidney and skin is used as an indicator of exposure to different environmental contaminants. This kind of analysis is a useful tool for assessment of habitat pollution levels particularly for sub-lethal and chronic effects. Standardised method according to Bernet et al. (1999) is applied for description and assessment of histological changes. According to this method the extension of a pathological change is rated with a “score value”, and the pathological importance of the alteration with an “importance factor”.

Biochemical analyses include determination of alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and alkaline phosphatase (AP) activity in sterlet blood sera, as well as concentration of total protein, urea and creatinine.

Results of histological and biochemical analysis will be used to determine if correlation between certain events exist or not: histopathological changes in liver and transaminase activity increase, histopathological changes of gills and urea concentrations, and histopathological changes of kidney and increase of creatinine concentrations.

Sterlet in investigated part of the Danube River is exposed to discharge of Belgrade industrial and domestic wastewater. Sterlet specimens were collected along the 50 km of the Danube River, the section which included localities in Belgrade and downstream. Comparative analysis of histopathological and biochemical parameters of sampled specimens in the vicinity of Belgrade waste water discharge sites, and specimens collected 50 km downstream, will be performed in order to explain possible effects of water pollution on investigated parameters.

 

Blood film analysis

Blood is one of the most assessable tissues, which gives you the opportunity to assess physiological and nutritional status without sacrificing the fish. Hematological indices commonly used by fish biologist (red blood cell count, hemoglobin concentration, blood hematocrit levels…) are adopted from human and veterinary medicine. Houston & Murad (1991) stated that fisheries biologist underscore a need for more detail characterization of red cell populations and that certain caution should be taken into a consideration during interpretation of hematological indices. The main reason for that is the fact that in healthy humans all circulating erythrocytes are mature while in fish, erythropoetic sites release cells to circulation at an early stage of development, which induce morphological heterogeneity in peripheral red cell population (Houston, 1997). That was the reason why Houston (1997) suggested an alternative way of measuring fish hematological status, called erythron profile. The description of the erythron profile involves estimation of relative abundance of various developmental stages found on blood smears. In our study we have applied method suggested by Houston (1997) in analysis of the influence of different water temperatures on morpholoogical characteristic of sterlet erythrocyte populations. Our goal is to fallow sterlet erythron profile during whole year cycle in attempt to determine standard values, which could be used later on as indicators of fish health. 

 

Parasitological analysis

Standard parasitological method was used to examine 260 sterlets (Acipenser ruthenus L.) so far. From all the sterlets examined 89.8% was infested. We established presence of 2 protozoa’s (Trichodina sp. and Ichthyophirius multiphilis) and 2 trematodes species (Skrjabinopsolus semiarmatus (Molin, 1956), Azygia robusta (Othner, 1911). Nematodes were present with two species (Contracaecum bidentatum (Linstow, 1899) and Contracaecum sinipercae Dogiel et Achmerov, 1959). Genus Acantocephala appeared with two species (Pomphorhynchus laevis (Müller, 1786) and Acanthocephalus lucii (Müller, 1786)). The most common parasite found was Skrjabinopsolus semiarmatus that was present with 14-360 specimens per fish and its prevalence was 78.57%. One to six specimens of Contracaecum sp. were found per fish as well, with the prevalence of 6.12%.

Analysis of the skin mucous film determined 100% prevalence of infestation with Trichodina sp. Other parasites were not so common and they were found only at few sterlet specimens.

Our investigation has showed that the degree of sterlet parasite infestation is very high and the further examinations are necessary to determine the role of parasites in regulation of starlet’s population density. This data can also be a good indicator of the extent of sterlet habitat pollution.

 

CONCLUSIONS

 

Sturgeon species have several aspects that make them important for protection and preservation. Apart from the fact that they make good scientific object (philogeneticaly very old group with different adaptation strategies) their economical value is very high as well. However there is a lack of recent and relevant data on natural populations of many sturgeons. One of those species is sterlet as well. It was especially noted by Reinartz (2002) that little is known about sterlet populations in the Lower Danube region. That is why we are trying to update existing data made by Jankovic (1958) and Ristic (1971) and to go further in investigation of life history aspects of this species. So far attention was given to morphology, physiology, histology, parasitology and ecology. In the future we would like to concentrate more on sterlet behavior in order to complete the knowledge on this species. Especially important are its migrations, which can excide more than 300 km (Hensel and Holcik, 1997) in the Danube River.

Hopefully our further knowledge, together with existing one, will provide us with a good base for resolving “two morphs” dilemma, which will then lead to appropriate, and adequate sterlet protection at the end.    

 

 

 

LITERATURE

 

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Houston A.H. & Murad A. (1991) Hematological characterization of goldfish, Carassius auratus L., by image analysis: effects of thermal acclimation and heat shock. Canadian Journal of Zoology, 69, 2041-2047.

 

Houston A.H. (1997) Review: Are the classical hematological variables acceptable indicators of fish health? Transactions of the American Fisheries Society, 126, 879-894.

 

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